Application

Western Blotting Analysis: 1 ug/mL from a representative lot detected TAZ in mouse lung tissue lysate.

Immunoprecipitation Analysis: A representative lot immunoprecipitated TAZ in Immunoprecipitation applications (Habbig, S., et. al. (2012). Hum Mol Genet. 21(26):5528-38).

Western Blotting Analysis: A representative lot detected TAZ in Western Blotting applications (Habbig, S., et. al. (2012). Hum Mol Genet. 21(26):5528-38).

ELISA Analysis: A representative lot detected TAZ in ELISA applications (Habbig, S., et. al. (2012). Hum Mol Genet. 21(26):5528-38).

Anti-TAZ, clone 11, Cat. No. MABS1913, is a mouse monoclonal antibody that detects WW domain-containing transcription regulator protein 1 (TAZ) and has been tested for use in ELISA, Immunoprecipitation, and Western Blotting.

General description

WW domain-containing transcription regulator protein 1 (UniProt: Q9EPK5; also known as Transcriptional coactivator with PDZ-binding motif, TAZ) is encoded by the Wwtr1 (also known as Taz) gene (Gene ID: 97064) in murine species. TAZ is an oncogenic transcriptional activator that acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. TAZ is highly expressed in kidney, heart, placenta, and lung tissues. Two isoforms of TAZ have been reported that are produced by alternative splicing. TAZ is concentrated along specific portions of the plasma membrane and also accumulates in punctate nuclear bodies. The ability of TAZ to regulate gene transcription is very tightly regulated through the phosphorylation-dependent binding of 14-3-3 protein. Upon phosphorylation TAZ is retained in cytoplasm by 14-3-3 zeta. Expression of NPHP9 is reported to inhibit the interaction of wild-type TAZ with 14-3-3 and NPHP9 is reported to abolish the phosphorylation of TAZ at Serine 89. TAZ is shown to be involved in non-small-cell lung cancer and its higher expression levels are observed in breast cancer and in breast cancer stem cells. It also regulates embryonic stem-cell self-renewal and promotes cell proliferation and epithelial-mesenchymal transition. TAZ binds to transcription factors via its WW domain, which is localized to amino acids 124-157. Its PZD-binding motif is located in amino acids 389-395 and this motif is essential for stimulated gene transcription.

Immunogen

His-tagged Recombinant Protein corresponding to Mouse WW domain-containing transcription regulator protein 1 (TAZ).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: 1 ug/mL of this antibody detected TAZ in mouse kidney tissue lysate.

Specificity

Clone 11 specifically detects WW domain-containing transcription regulator protein 1 (TAZ) in murine species.

Target description

~55 kDa observed; 43.62 and 49.83 kDa for isoform 1 and 2, respectively. Uncharacterized bands may be observed in some lysate(s).